Continued from H.10B biotechnology or pharmaceutical industry.br br Experience may be gained concurrently and must includebr br Two 2 years in each of the followingbr ELISA, ELISpot, mammalian cell culture including tumor cell lines and primary immune cells, WES, qPCR, immunophenotyping, flow cytometry, and multicolor panel designbr Designing and developing engineered cell lines, including Tcell activation and proliferation assays, using CRISPR technology, and generating CARTcellsbr Managing and executing in vivo mouse studies, including animal handling, performing routine injections SC, IV, PO, and IP, necropsy, and collection of tissues and blood from various routes such as submandibular or facial vein, tail vein, open or closed cardiac collectionbr Working on crossfunctional engagementsbr Developing technical documentation, including SOPs, preclinical IND package, and QC reportsbr Establishing in vivo syngeneic and xenograft tumor models, including transgenic or knockout micebr Animal study management software Studylog, flow cytometry data analysis software FlowJo, DNA cloning software such as SnapGene, and image processing program such as ImageJbr br Employer will accept any suitable combination of education, training and experience.
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