10.B Continuedbr The occupation must include 3 years of experience with enzymatic assay development. Experience must include the followingbr Enzymatic assay development with cell lysates and purified proteinsbr Handson experience in biochemical methods, including SDSPAGE, Western Blot, and immunoprecipitationbr Biochemical assays, including in CRISPR KOKI, siRNA KOKI, iPSC culture and differentiating iPSCs into different Cell typebr Mammalian cell culture and basic cell biology techniques, including transfectionsbr Executing experiments, analyzing, and interpreting results with minimal supervision.br br We will alternatively accept a bachelors degree and 6 years of stated experience.br br Q.H.12 PLEASE NOTE THAT THE REQUIREMENTS FOR THIS POSITION ARE SUPPORTED BY BUSINESS NECESSITY. The requirements of a Masters degree and 3 years of experience or a Bachelors degree and 6 years of experience bear a reasonable relationship to the occupation of Biochemists and Biophysicists in the context of the research and development in biotechnology field. Specifically, the nature of this position requires someone with proficient experience in enzyme assays and with the ability to develop biochemical assays to evaluate the activity of novel E3 ligases.br br PLEASE NOTE There is a typographical error on the Prevailing Wage Determination in part F.d.e.1. the worksite is listed as quot;Associate Scientist, Platform Biologyquot; and should have been quot;200 Arsenal Yards Blvdquot;. The lines 2 6 are correct and this typographical error is not material to the Prevailing Wage Determination since both addresses are listed as being within Watertown City. Area Code 71650 for Area Name BostonCambridgeNashua, MANH.

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